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Stochastic transcription in the p53-mediated response to DNA damage is modulated by burst frequency

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Item Type:Article
Title:Stochastic transcription in the p53-mediated response to DNA damage is modulated by burst frequency
Creators Name:Friedrich, D. and Friedel, L. and Finzel, A. and Herrmann, A. and Preibisch, S. and Loewer, A.
Abstract:Discontinuous transcription has been described for different mammalian cell lines and numerous promoters. However, our knowledge of how the activity of individual promoters is adjusted by dynamic signaling inputs from transcription factors is limited. To address this question, we characterized the activity of selected target genes that are regulated by pulsatile accumulation of the tumor suppressor p53 in response to ionizing radiation. We performed time-resolved measurements of gene expression at the single-cell level by smFISH and used the resulting data to inform a mathematical model of promoter activity. We found that p53 target promoters are regulated by frequency modulation of stochastic bursting and can be grouped along three archetypes of gene expression. The occurrence of these archetypes cannot solely be explained by nuclear p53 abundance or promoter binding of total p53. Instead, we provide evidence that the time-varying acetylation state of p53's C-terminal lysine residues is critical for gene-specific regulation of stochastic bursting.
Keywords:Cellular Heterogeneity, DNA Damage, p53 Signaling, Single-Cell Analysis, Stochastic Transcription
Source:Molecular Systems Biology
ISSN:1744-4292
Publisher:EMBO Press
Volume:15
Number:12
Page Range:e9068
Date:1 December 2019
Official Publication:https://doi.org/10.15252/msb.20199068
PubMed:View item in PubMed

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