RIM-BP2 primes synaptic vesicles via recruitment of Munc13-1 at hippocampal mossy fiber synapses

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Item Type:

Article

Title:

RIM-BP2 primes synaptic vesicles via recruitment of Munc13-1 at hippocampal mossy fiber synapses

Creators Name:

Brockmann, M.M. and Maglione, M. and Willmes, C.G. and Stumpf, A. and Bouazza, B.A. and Velasquez, L.M. and Grauel, M.K. and Beed, P. and Lehmann, M. and Gimber, N. and Schmoranzer, J. and Sigrist, S.J. and Rosenmund, C. and Schmitz, D.

Abstract:

All synapses require fusion-competent vesicles and coordinated Ca(2+)-secretion coupling for neurotransmission, yet functional and anatomical properties are diverse across different synapse types. We show that the presynaptic protein RIM-BP2 has diversified functions in neurotransmitter release at different central murine synapses and thus contributes to synaptic diversity. At hippocampal pyramidal CA3-CA1 synapses, RIM-BP2 loss has a mild effect on neurotransmitter release, by only regulating Ca(2+)-secretion coupling. However, at hippocampal mossy fiber synapses, RIM-BP2 has a substantial impact on neurotransmitter release by promoting vesicle docking/priming and vesicular release probability via stabilization of Munc13-1 at the active zone. We suggest that differences in the active zone organization may dictate the role a protein plays in synaptic transmission and that differences in active zone architecture is a major determinant factor in the functional diversity of synapses.

Keywords:

Hippocampal Mossy Fibers, Nerve Tissue Proteins, Neurons, Neurotransmitter Agents, Synaptic Vesicles, Animals, Mice

Source:

eLife

ISSN:

2050-084X

Publisher:

eLife Sciences Publications

Volume:

Page Range:

e43243

Date: