Multiple two-photon targeted whole-cell patch-clamp recordings from monosynaptically connected neurons in vivo

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Item Type:

Article

Title:

Multiple two-photon targeted whole-cell patch-clamp recordings from monosynaptically connected neurons in vivo

Creators Name:

Jouhanneau, J.S. and Poulet, J.F.A.

Abstract:

Although we know a great deal about monosynaptic connectivity, transmission and integration in the mammalian nervous system from in vitro studies, very little is known in vivo. This is partly because it is technically difficult to evoke action potentials and simultaneously record small amplitude subthreshold responses in closely (<150 µm) located pairs of neurons. To address this, we have developed in vivo two-photon targeted multiple (2–4) whole-cell patch clamp recordings of nearby neurons in superficial cortical layers 1–3. Here, we describe a step-by-step guide to this approach in the anesthetized mouse primary somatosensory cortex, including: the design of the setup, surgery, preparation of pipettes, targeting and acquisition of multiple whole-cell recordings, as well as in vivo and post hoc histology. The procedure takes ∼4 h from start of surgery to end of recording and allows examinations both into the electrophysiological features of unitary excitatory and inhibitory monosynaptic inputs during different brain states as well as the synaptic mechanisms of correlated neuronal activity.

Keywords:

Cortex, Synapse, Whole-Cell, Two-Photon Microscopy, In Vivo, Animals, Mice

Source:

Frontiers in Synaptic Neuroscience

ISSN:

1663-3563

Publisher:

Frontiers Media SA

Volume:

Page Range:

Date:

May 2019