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Postnatal DNA demethylation and its role in tissue maturation.

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Item Type:Article
Title:Postnatal DNA demethylation and its role in tissue maturation.
Creators Name:Reizel, Y. and Sabag, O. and Skversky, Y. and Spiro, A. and Steinberg, B. and Bernstein, D. and Wang, A. and Kieckhaefer, J. and Li, C. and Pikarsky, E. and Levin-Klein, R. and Goren, A. and Rajewsky, K. and Kaestner, K.H. and Cedar, H.
Abstract:Development in mammals is accompanied by specific de novo and demethylation events that are thought to stabilize differentiated cell phenotypes. We demonstrate that a large percentage of the tissue-specific methylation pattern is generated postnatally. Demethylation in the liver is observed in thousands of enhancer-like sequences associated with genes that undergo activation during the first few weeks of life. Using. conditional gene ablation strategy we show that the removal of these methyl groups is stable and necessary for assuring proper hepatocyte gene expression and function through its effect on chromatin accessibility. These postnatal changes in methylation come about through exposure to hormone signaling. These results define the molecular rules of 5-methyl-cytosine regulation as an epigenetic mechanism underlying cellular responses to. changing environment.
Keywords:5-Methylcytosine, Cultured Cells, Developmental Gene Expression Regulation, DNA Demethylation, DNA-Binding Proteins, Genetic Epigenesis, Hepatocytes, High-Throughput Nucleotide Sequencing, Inbred C57BL Mice, Knockout Mice, Liver, Newborn Animals, Primary Cell Culture, Proto-Oncogene Proteins, RNA Sequence Analysis, Signal Transduction, Animals, Mice
Source:Nature Communications
Publisher:Nature Publishing Group
Page Range:2040
Date:23 May 2018
Official Publication:https://doi.org/10.1038/s41467-018-04456-6
PubMed:View item in PubMed

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