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A single-cell transcriptome atlas of the mouse glomerulus

Item Type:Article
Title:A single-cell transcriptome atlas of the mouse glomerulus
Creators Name:Karaiskos, N. and Rahmatollahi, M. and Boltengagen, A. and Liu, H. and Hoehne, M. and Rinschen, M. and Schermer, B. and Benzing, T. and Rajewsky, N. and Kocks, C. and Kann, M. and Müller, R.U.
Abstract:BACKGROUND: Three different cell types constitute the glomerular filter: mesangial cells, endothelial cells, and podocytes. However, to what extent cellular heterogeneity exists within healthy glomerular cell populations remains unknown. METHODS: We used nanodroplet-based highly parallel transcriptional profiling to characterize the cellular content of purified wild-type mouse glomeruli. RESULTS: Unsupervised clustering of nearly 13,000 single-cell transcriptomes identified the three known glomerular cell types. We provide a comprehensive online atlas of gene expression in glomerular cells that can be queried and visualized using an interactive and freely available database. Novel marker genes for all glomerular cell types were identified and supported by immunohistochemistry images obtained from the Human Protein Atlas. Subclustering of endothelial cells revealed a subset of endothelium that expressed marker genes related to endothelial proliferation. By comparison, the podocyte population appeared more homogeneous but contained three smaller, previously unknown subpopulations. CONCLUSIONS: Our study comprehensively characterized gene expression in individual glomerular cells and sets the stage for the dissection of glomerular function at the single-cell level in health and disease.
Keywords:Glomerulus, Single-Cell RNA Sequencing, scRNAseq, Podocyte, Transcriptome, Animals, Mice
Source:Journal of the American Society of Nephrology
Publisher:American Society of Nephrology
Page Range:2060-2068
Date:August 2018
Official Publication:https://doi.org/10.1681/ASN.2018030238
External Fulltext:View full text on PubMed Central
PubMed:View item in PubMed

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