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| Item Type: | Article |
|---|---|
| Title: | Dynamic stroma reorganization drives blood vessel dysmorphia during glioma growth |
| Creators Name: | Mathivet, T. and Bouleti, C. and Van Woensel, M. and Stanchi, F. and Verschuere, T. and Phng, L.K. and Dejaegher, J. and Balcer, M. and Matsumoto, K. and Georgieva, P.B. and Belmans, J. and Sciot, R. and Stockmann, C. and Mazzone, M. and De Vleeschouwer, S. and Gerhardt, H. |
| Abstract: | Glioma growth and progression are characterized by abundant development of blood vessels that are highly aberrant and poorly functional, with detrimental consequences for drug delivery efficacy. The mechanisms driving this vessel dysmorphia during tumor progression are poorly understood. Using longitudinal intravital imaging in a mouse glioma model, we identify that dynamic sprouting and functional morphogenesis of a highly branched vessel network characterize the initial tumor growth, dramatically changing to vessel expansion, leakage, and loss of branching complexity in the later stages. This vascular phenotype transition was accompanied by recruitment of predominantly pro-inflammatory M1-like macrophages in the early stages, followed by in situ repolarization to M2-like macrophages, which produced VEGF-A and relocate to perivascular areas. A similar enrichment and perivascular accumulation of M2 versus M1 macrophages correlated with vessel dilation and malignancy in human glioma samples of different WHO malignancy grade. Targeting macrophages using anti-CSF1 treatment restored normal blood vessel patterning and function. Combination treatment with chemotherapy showed survival benefit, suggesting that targeting macrophages as the key driver of blood vessel dysmorphia in glioma progression presents opportunities to improve efficacy of chemotherapeutic agents. We propose that vessel dysfunction is not simply a general feature of tumor vessel formation, but rather an emergent property resulting from a dynamic and functional reorganization of the tumor stroma and its angiogenic influences. |
| Keywords: | Glioma, Live Imaging, Myeloid Cells, VEGF, Vessel Dysmorphia, Animals, Mice |
| Source: | EMBO Molecular Medicine |
| ISSN: | 1757-4676 |
| Publisher: | EMBO Press / Wiley |
| Volume: | 9 |
| Number: | 12 |
| Page Range: | 1629-1645 |
| Date: | 1 December 2017 |
| Official Publication: | https://doi.org/10.15252/emmm.201607445 |
| PubMed: | View item in PubMed |
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