Gene therapy with the Sleeping Beauty transposon system

Item Type:	Review
Title:	Gene therapy with the Sleeping Beauty transposon system
Creators Name:	Kebriaei, P. and Izsvák, Z. and Narayanavari, S.A. and Singh, H. and Ivics, Z.
Abstract:	The widespread clinical implementation of gene therapy requires the ability to stably integrate genetic information through gene transfer vectors in a safe, effective, and economical manner. The latest generation of Sleeping Beauty (SB) transposon vectors fulfills these requirements, and may overcome limitations associated with viral gene transfer vectors and transient nonviral gene delivery approaches that are prevalent in ongoing clinical trials. The SB system enables high-level stable gene transfer and sustained transgene expression in multiple primary human somatic cell types, thereby representing a highly attractive gene transfer strategy for clinical use. Here, we review the most important aspects of using SB for gene therapy, including vectorization as well as genomic integration features. We also illustrate the path to successful clinical implementation by highlighting the application of chimeric antigen receptor (CAR)-modified T cells in cancer immunotherapy.
Keywords:	Gene Therapy Vectors, Chimeric Antigen Receptor, Stable Gene Transfer, Non-Viral Gene Delivery, Transposon/Virus Hybrid Vectors, Cell-Specific Targeting, Animals, Mice
Source:	Trends in Genetics
ISSN:	0168-9525
Publisher:	Elsevier
Volume:	33
Number:	11
Page Range:	852-870
Date:	November 2017
Official Publication:	https://doi.org/10.1016/j.tig.2017.08.008
PubMed:	View item in PubMed

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