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In vivo interaction proteomics in Caenorhabditis elegans embryos provides new insights into P granule dynamics

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Item Type:Article
Title:In vivo interaction proteomics in Caenorhabditis elegans embryos provides new insights into P granule dynamics
Creators Name:Chen, J.X. and Cipriani, P.G. and Mecenas, D. and Polanowska, J. and Piano, F. and Gunsalus, K.C. and Selbach, M.
Abstract:Studying protein interactions in whole organisms is fundamental to understanding development. Here, we combine in vivo expressed GFP-tagged proteins with quantitative proteomics to identify protein-protein interactions of selected key proteins involved in early C. elegans embryogenesis. Co-affinity purification of interaction partners for eight bait proteins resulted in a pilot in vivo interaction map of proteins with a focus on early development. Our network reflects known biology and is highly enriched in functionally relevant interactions. To demonstrate the utility of the map, we looked for new regulators of P granule dynamics and found that GEI-12, a novel binding partner of the DYRK family kinase MBK-2, is a key regulator of P granule formation and germline maintenance. Our data corroborate a recently proposed model in which the phosphorylation state of GEI-12 controls P granule dynamics. In addition, we find that GEI-12 also induces granule formation in mammalian cells, suggesting a common regulatory mechanism in worms and humans. Our results show that in vivo interaction proteomics provides unique insights into animal development.
Keywords:Affinity Chromatography, Caenorhabditis elegans Proteins, Carrier Proteins, Cytoplasmic Granules, Developmental Gene Expression Regulation, Mass Spectrometry, Phosphorylation, Protein Interaction Maps, Protein-Serine-Threonine Kinases, Protein-Tyrosine Kinases, Proteomics, Animals, Caenorhabditis elegans
Source:Molecular & Cellular Proteomics
Publisher:American Society for Biochemistry and Molecular Biology
Page Range:1642-1657
Date:1 May 2016
Official Publication:https://doi.org/10.1074/mcp.M115.053975
PubMed:View item in PubMed

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