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Identification of molecular compartments and genetic circuitry in the developing mammalian kidney

Item Type:Article
Title:Identification of molecular compartments and genetic circuitry in the developing mammalian kidney
Creators Name:Yu, J. and Valerius, M.T. and Duah, M. and Staser, K. and Hansard, J.K. and Guo, J.J. and McMahon, J. and Vaughan, J. and Faria, D. and Georgas, K. and Rumballe, B. and Ren, Q. and Krautzberger, A.M. and Junker, J.P. and Thiagarajan, R.D. and Machanick, P. and Gray, P.A. and van Oudenaarden, A. and Rowitch, D.H. and Stiles, C.D. and Ma, Q. and Grimmond, S.M. and Bailey, T.L. and Little, M.H. and McMahon, A.P.
Abstract:Lengthy developmental programs generate cell diversity within an organotypic framework, enabling the later physiological actions of each organ system. Cell identity, cell diversity and cell function are determined by cell type-specific transcriptional programs; consequently, transcriptional regulatory factors are useful markers of emerging cellular complexity, and their expression patterns provide insights into the regulatory mechanisms at play. We performed a comprehensive genome-scale in situ expression screen of 921 transcriptional regulators in the developing mammalian urogenital system. Focusing on the kidney, analysis of regional-specific expression patterns identified novel markers and cell types associated with development and patterning of the urinary system. Furthermore, promoter analysis of synexpressed genes predicts transcriptional control mechanisms that regulate cell differentiation. The annotated informational resource (www.gudmap.org) will facilitate functional analysis of the mammalian kidney and provides useful information for the generation of novel genetic tools to manipulate emerging cell populations.
Keywords:Genome-scale Expression Screen, Transcriptional Regulator, Kidney, Mouse, Animals, Mice
Publisher:Company of Biologists
Page Range:1863-1873
Date:May 2012
Official Publication:https://doi.org/10.1242/dev.074005
PubMed:View item in PubMed

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