Circular RNAs in the mammalian brain are highly abundant, conserved, and dynamically expressed

Item Type:	Article
Title:	Circular RNAs in the mammalian brain are highly abundant, conserved, and dynamically expressed
Creators Name:	Rybak-Wolf, A. and Stottmeister, C. and Glažar, P. and Jens, M. and Pino, N. and Giusti, S. and Hanan, M. and Behm, M. and Bartok, O. and Ashwal-Fluss, R. and Herzog, M. and Schreyer, L. and Papavasileiou, P. and Ivanov, A. and Öhman, M. and Refojo, D. and Kadener, S. and Rajewsky, N.
Abstract:	Circular RNAs (circRNAs) are an endogenous class of animal RNAs. Despite their abundance, their function and expression in the nervous system are unknown. Therefore, we sequenced RNA from different brain regions, primary neurons, isolated synapses, as well as during neuronal differentiation. Using these and other available data, we discovered and analyzed thousands of neuronal human and mouse circRNAs. circRNAs were extraordinarily enriched in the mammalian brain, well conserved in sequence, often expressed as circRNAs in both human and mouse, and sometimes even detected in Drosophila brains. circRNAs were overall upregulated during neuronal differentiation, highly enriched in synapses, and often differentially expressed compared to their mRNA isoforms. circRNA expression correlated negatively with expression of the RNA-editing enzyme ADAR1. Knockdown of ADAR1 induced elevated circRNA expression. Together, we provide a circRNA brain expression atlas and evidence for important circRNA functions and values as biomarkers.
Keywords:	Base Sequence, Brain, Cell Line, DNA Sequence Analysis, Messenger RNA, Molecular Sequence Data, Neurogenesis, Organ Specificity, RNA, Synapses, Animals, Drosophila melanogaster, Mice
Source:	Molecular Cell
ISSN:	1097-2765
Publisher:	Cell Press / Elsevier
Volume:	58
Number:	5
Page Range:	870-885
Date:	4 June 2015
Official Publication:	https://doi.org/10.1016/j.molcel.2015.03.027
PubMed:	View item in PubMed

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