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| Item Type: | Article |
|---|---|
| Title: | Argonaute2 regulates the pancreatic β-cell secretome |
| Creators Name: | Tattikota, S.G. and Sury, M.D. and Rathjen, T. and Wessels, H.H. and Pandey, A.K. and You, X. and Becker, C. and Chen, W. and Selbach, M. and Poy, M.N. |
| Abstract: | Argonaute2 (Ago2) is an established component of the microRNA-induced silencing complex. Similar to miR-375 loss-of-function studies, inhibition of Ago2 in the pancreatic beta-cell resulted in enhanced insulin release underlining the relationship between these two genes. Moreover, as the most abundant microRNA in pancreatic endocrine cells, miR-375 was also observed to be enriched in Ago2-associated complexes. Both Ago2 and miR-375 regulate the pancreatic beta-cell secretome and we identified using quantitative mass spectrometry the enhanced release of a set of proteins or secretion signature in response to a glucose stimulus using the murine beta-cell line, MIN6. In addition, loss of Ago2 resulted in the increased expression of miR-375 target genes, including gephyrin and ywhaz. These targets positively contribute to exocytosis indicating they may mediate the functional role of both miR-375 and Ago proteins in the pancreatic beta-cell by influencing the secretory pathway. This study specifically addresses the role of Ago2 in the systemic release of proteins from beta-cells and highlights the contribution of the microRNA pathway to the function of this cell type. |
| Keywords: | Argonaute Proteins, Cell Line, Gene Expression Regulation, Insulin, Insulin-Secreting Cells, MicroRNAs, Proteome, RNA Interference, Animals, Mice |
| Source: | Molecular & Cellular Proteomics |
| ISSN: | 1535-9476 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Volume: | 12 |
| Number: | 5 |
| Page Range: | 1214-1225 |
| Date: | May 2013 |
| Official Publication: | https://doi.org/10.1074/mcp.M112.024786 |
| PubMed: | View item in PubMed |
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