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Viral microRNA targetome of KSHV-infected primary effusion lymphoma cell lines

Item Type:Article
Title:Viral microRNA targetome of KSHV-infected primary effusion lymphoma cell lines
Creators Name:Gottwein, E. and Corcoran, D.L. and Mukherjee, N. and Skalsky, R.L. and Hafner, M. and Nusbaum, J.D. and Shamulailatpam, P. and Love, C.L. and Dave, S.S. and Tuschl, T. and Ohler, U. and Cullen, B.R.
Abstract:Primary effusion lymphoma (PEL) is caused by Kaposis sarcoma-associated herpesvirus (KSHV) and frequently also harbors Epstein-Barr virus (EBV). The expression of KSHV- and EBV-encoded microRNAs (miRNAs) in PELs suggests a role for these miRNAs in latency and lymphomagenesis. Using PAR-CLIP, a technology which allows the direct and transcriptome-wide identification of miRNA targets, we delineate the target sites for all viral and cellular miRNAs expressed in PEL cell lines. The resulting data set revealed that KSHV miRNAs directly target more than 2000 cellular mRNAs, including many involved in pathways relevant to KSHV pathogenesis. Moreover, 58% of these mRNAs are also targeted by EBV miRNAs, via distinct binding sites. In addition to a known viral analog of cellular miR-155, we show that KSHV encodes a viral miRNA that mimics cellular miR-142-3p function. In summary, this study identifies an extensive list of KSHV miRNA targets, which are likely to influence viral replication and pathogenesis.
Keywords:Base Sequence, Human Herpesvirus 8, MicroRNAs, Molecular Sequence Data, Primary Effusion Lymphoma, Tumor Cell Line, Viral RNA
Source:Cell Host & Microbe
Page Range:515-526
Date:17 November 2011
Official Publication:https://doi.org/10.1016/j.chom.2011.09.012
PubMed:View item in PubMed

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