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The sleeping beauty transposon toolbox

Item Type:Article
Title:The sleeping beauty transposon toolbox
Creators Name:Ammar, I. and Izsvak, Z. and Ivics, Z.
Abstract:The mobility of class II transposable elements (DNA transposons) can be experimentally controlled by separating the two functional components of the transposon: the terminal inverted repeat sequences that flank a gene of interest to be mobilized and the transposase protein that can be conditionally supplied to drive the transposition reaction. Thus, a DNA molecule of interest (e.g., a fluorescent marker, an shRNA expression cassette, a mutagenic gene trap or a therapeutic gene construct) cloned between the inverted repeat sequences of a transposon-based vector can be stably integrated into the genome in a regulated and highly efficient manner. Sleeping Beauty (SB) was the first transposon ever shown capable of gene transfer in vertebrate cells, and recent results confirm that SB supports a full spectrum of genetic engineering in vertebrate species, including transgenesis, insertional mutagenesis, and therapeutic somatic gene, transfer both ex vivo and in vivo. This methodological paradigm opened up a number of avenues for genome manipulations for basic and applied research. This review highlights the state-of-the-art in SB transposon technology in diverse genetic applications with special emphasis on the transposon as well as transposase vectors currently available in the SB transposon toolbox.
Keywords:Mutagenesis, Gene Transfer, Transgenesis, Gene Therapy, Transposase, Inverted Repeats, Gene Vectors, Animals
Source:Methods in Molecular Biology
Publisher:Springer / Humana Press
Page Range:229-240
Date:31 January 2012
Official Publication:https://doi.org/10.1007/978-1-61779-603-6_13
PubMed:View item in PubMed

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