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The hyperactive Sleeping Beauty transposase SB100X improves the genetic modification of T cells to express a chimeric antigen receptor

Item Type:Article
Title:The hyperactive Sleeping Beauty transposase SB100X improves the genetic modification of T cells to express a chimeric antigen receptor
Creators Name:Jin, Z. and Maiti, S. and Huls, H. and Singh, H. and Olivares, S. and Mates, L. and Izsvak, Z. and Ivics, Z. and Lee, D.A. and Champlin, R.E. and Cooper, L.J.
Abstract:Sleeping Beauty (SB(3)) transposon and transposase constitute a DNA plasmid system used for therapeutic human cell genetic engineering. Here we report a comparison of SB100X, a newly developed hyperactive SB transposase, to a previous generation SB11 transposase to achieve stable expression of a CD19-specific chimeric antigen receptor (CAR(3)) in primary human T cells. The electro-transfer of SB100X expressed from a DNA plasmid or as an introduced mRNA species had superior transposase activity in T cells based on the measurement of excision circles released after transposition and emergence of CAR expression on T cells selectively propagated upon CD19(+) artificial antigen-presenting cells. Given that T cells modified with SB100X and SB11 integrate on average one copy of the CAR transposon in each T-cell genome, the improved transposition mediated by SB100X apparently leads to an augmented founder effect of electroporated T cells with durable integration of CAR. In aggregate, SB100X improves SB transposition in primary human T cells and can be titrated with an SB transposon plasmid to improve the generation of CD19-specific CAR(+) T cells.
Keywords:Chimeric Antigen Receptor, T Cells, Sleeping Beauty, SB11, SB100X, CD19
Source:Gene Therapy
ISSN:0969-7128
Publisher:Nature Publishing Group
Volume:18
Number:9
Page Range:849-856
Date:September 2011
Official Publication:https://doi.org/10.1038/gt.2011.40
PubMed:View item in PubMed

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