Generating knockout rats by transposon mutagenesis in spermatogonial stem cells

Item Type:	Article
Title:	Generating knockout rats by transposon mutagenesis in spermatogonial stem cells
Creators Name:	Izsvak, Z. and Froehlich, J. and Grabundzija, I. and Shirley, J.R. and Powell, H.M. and Chapman, K.M. and Ivics, Z. and Hamra, F.K.
Abstract:	Disrupting genes in the rat on a genome-wide scale will allow the investigation of many biological processes linked to human health. Here we used transposon-mediated mutagenesis to knock out genes in rat spermatogonial stem cells. Given the capacity of the testis to support spermatogenesis from thousands of transplanted, genetically manipulated spermatogonia, this approach paves a way for high-throughput functional genomic studies in the laboratory rat.
Keywords:	DNA Transposable Elements, Gene Knockout Techniques, Mutagenesis, Spermatogonia, Stem Cells, Animals, Rats
Source:	Nature Methods
ISSN:	1548-7091
Publisher:	Nature Publishing Group
Volume:	7
Number:	6
Page Range:	443-445
Date:	June 2010
Official Publication:	https://doi.org/10.1038/nmeth.1461
PubMed:	View item in PubMed

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