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Vegf as an epithelial cell morphogen modulates branching morphogenesis of embryonic kidney by directly acting on the ureteric bud

Item Type:Article
Title:Vegf as an epithelial cell morphogen modulates branching morphogenesis of embryonic kidney by directly acting on the ureteric bud
Creators Name:Marlier, A. and Schmidt-Ott, K.M. and Gallagher, A.R. and Barasch, J. and Karihaloo, A.
Abstract:There is growing evidence that vascular endothelial growth factor (Vegf), a well-recognized angiogenic factor, plays a regulatory role in non-endothelial tissues such as neurons and epithelial cells. In the kidney Vegf receptors have been detected in proximal tubule cells of the adult kidney and Vegf has been show to stimulate branching morphogenesis of the developing kidney. In this study, using laser-microdissection as well as manual separation of the UB, we demonstrate that Vegf receptors are present in the ureteric bud (UB). Furthermore, we determine that Vegf stimulates UB branching in whole kidney explant that is mediated directly by signaling through Vegfr2. In addition, Vegf also induced branching response in isolated UBs that are free of the surrounding mesenchyme. These responses seem to be strictly dependent on the dose of Vegf such that higher doses are inhibitory while lower dose are stimulatory. These data place Vegf in a unique position of being able to modulate vascular as well as epithelial development in the embryonic kidney.
Keywords:Ureteric-Bud, Branching Morphogenesis, Flk1, Vegf, Epithelial Cells, Kidney, Kidney Tubules, Organ Culture Techniques, Sprague-Dawley Rats, Ureter, Animals, Mice, Rats
Source:Mechanisms of Development
ISSN:0925-4773
Publisher:Elsevier (The Netherlands)
Volume:126
Number:3-4
Page Range:91-98
Date:March 2009
Official Publication:https://doi.org/10.1016/j.mod.2008.12.003
PubMed:View item in PubMed

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