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Positioning of subdomain IIId and apical loop of domain II of the hepatitis C IRES on the human 40S ribosome

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Item Type:Article
Title:Positioning of subdomain IIId and apical loop of domain II of the hepatitis C IRES on the human 40S ribosome
Creators Name:Babaylova, E. and Graifer, D. and Malygin, A. and Stahl, J. and Shatsky, I. and Karpova, G.
Abstract:The 5'-untranslated region of the hepatitis C virus (HCV) RNA contains a highly structured motif called IRES (Internal Ribosome Entry Site) responsible for the cap-independent initiation of the viral RNA translation. At first, the IRES binds to the 40S subunit without any initiation factors so that the initiation AUG codon falls into the P site. Here using an original site-directed cross-linking strategy, we identified 40S subunit components neighboring subdomain IIId, which is critical for HCV IRES binding to the subunit, and apical loop of domain II, which was suggested to contact the 40S subunit from data on cryo-electron microscopy of ribosomal complexes containing the HCV IRES. HCV IRES derivatives that bear a photoactivatable group at nucleotide A275 or at G263 in subdomain IIId cross-link to ribosomal proteins S3a, S14 and S16, and HCV IRES derivatized at the C83 in the apex of domain II cross-link to proteins S14 and S16.
Keywords:5' Untranslated Regions, Base Sequence, Hepacivirus, Molecular Sequence Data, Nucleic Acid Conformation, Translational Peptide Chain Initiation, Viral RNA, Ribosomal Proteins, Eukaryotic Small Ribosome Subunits, Ultraviolet Rays
Source:Nucleic Acids Research
ISSN:0305-1048
Publisher:Oxford University Press (U.K.)
Volume:37
Number:4
Page Range:1141-1151
Date:March 2009
Official Publication:https://doi.org/10.1093/nar/gkn1026
PubMed:View item in PubMed

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