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Enhanced resolution of sedimentation coefficient distribution profiles by extrapolation to infinite time

Item Type:Article
Title:Enhanced resolution of sedimentation coefficient distribution profiles by extrapolation to infinite time
Creators Name:Behlke, J. and Ristau, O.
Abstract:Sedimentation velocity experiments can be used to identify two or more independent non-interacting macromolecules, which differ in their size by only a few percent. The procedure requires the extrapolation of differential apparent sedimentation coefficient distributions obtained at different running time to t --> infinity and works because it eliminates or greatly reduces diffusion effects. Here, we present an improved time extrapolation function of sedimentation distribution profiles originally presented by Stafford (In: Harding, Rowe, Horton (eds.) Analytical ultracentrifugation in biochemistry and polymer science, 1992). We describe a computing procedure with the program LAMM: to analyze concentration profiles obtained by absorbance or interference optics that utilizes suitable smoothing methods for noisy data sets and present examples which include time invariant noises.
Keywords:Computer Simulation, Sedimentation Coefficient Distribution, Recognition of Non-Interacting Macromolecules
Source:European Biophysics Journal
ISSN:0175-7571
Publisher:Springer (Germany)
Volume:39
Number:3
Page Range:449-455
Date:February 2010
Official Publication:https://doi.org/10.1007/s00249-009-0425-1
PubMed:View item in PubMed

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