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Probing the electronic structure of the hemoglobin active center in physiological solutions

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Item Type:Article
Title:Probing the electronic structure of the hemoglobin active center in physiological solutions
Creators Name:Aziz, E.F. and Ottosson, N. and Bonhommeau, S. and Bergmann, N. and Eberhardt, W. and Chergui, M.
Abstract:Soft-x-ray absorption spectroscopy at the L_{2,3} edge of the iron center in bovine hemoglobin and hemin under physiological conditions is reported for the first time. Spectra of the same compounds in solid form are presented for comparison. Striking differences in the electronic structure of the metalloporphyrin are observed between the liquid and solid compounds. We unambiguously show that hemoglobin and hemin are in a high-spin ferric state in solution, and that the 2p spin-orbit coupling decreases for hemin compared to the hemoglobin, while this is not the case in solids. The spectra were simulated using ligand field multiplet theory, in good agreement with the experiment, allowing quantification of the amount of charge transfer between the porphyrin and Fe3+ ion in hemoglobin and in hemin.
Keywords:Catalytic Domain, Ferric Compounds, Hemin, Hemoglobins, Molecular Models, Solutions, X-Ray Emission Spectrometry, Animals, Cattle
Source:Physical Review Letters
ISSN:0031-9007
Publisher:American Physical Society (U.S.A.)
Volume:102
Number:6
Page Range:068103
Date:13 February 2009
Official Publication:https://doi.org/10.1103/PhysRevLett.102.068103
PubMed:View item in PubMed

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