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BK channels in innate immune functions of neutrophils and macrophages

Official URL:https://doi.org/10.1182/blood-2008-07-166660
PubMed:View item in PubMed
Creators Name:Essin, K. and Gollasch, M. and Rolle, S. and Weissgerber, P. and Sausbier, M. and Bohn, E. and Autenrieth, I.B. and Ruth, P. and Luft, F.C. and Nauseef, W.M. and Kettritz, R.
Journal Title:Blood
Journal Abbreviation:Blood
Page Range:1326-1331
Date:5 February 2009
Keywords:1-Phosphatidylinositol 3-Kinase, Flow Cytometry, Innate Immunity, Indoles, Large-Conductance Calcium-Activated Potassium Channels, Lipopolysaccharides, Macrophages, Mitogen-Activated Protein Kinase 1, Mitogen-Activated Protein Kinase 3, Vascular Smooth Muscle, NADPH Oxidase, NF-kappa B, Neutrophils, Phagocytes, Proto-Oncogene Proteins c-akt, Respiratory Burst, Signal Transduction, Superoxides, Tibial Arteries, Tumor Necrosis Factor-alpha, p38 Mitogen-Activated Protein Kinases, Animals, Mice
Abstract:Oxygen-dependent antimicrobial activity of human polymorphonuclear leukocytes (PMNs) relies on the phagocyte nicotinamide adenine dinucleotide phosphate (NADPH) oxidase to generate oxidants. As the oxidase transfers electrons from NADPH the membrane will depolarize and concomitantly terminate oxidase activity, unless there is charge translocation to compensate. Most experimental data implicate proton channels as the effectors of this charge compensation, although large-conductance Ca2+-activated K+ (BK) channels have been suggested to be essential for normal PMN antimicrobial activity. To test this latter notion, we directly assessed the role of BK channels in phagocyte function, including the NADPH oxidase. PMNs genetically lacking BK channels (BK(-/-)) had normal intracellular and extracellular NADPH oxidase activity in response to both receptor-independent and phagocytic challenges. Furthermore, NADPH oxidase activity of human PMNs and macrophages was normal after treatment with BK channel inhibitors. Although BK channel inhibitors suppressed endotoxin-mediated tumor necrosis factor-alpha secretion by bone marrow-derived macrophages (BMDMs), BMDMs of BK(-/-) and wild-type mice responded identically and exhibited the same ERK, PI3K/Akt, and nuclear factor-kappaB activation. Based on these data, we conclude that the BK channel is not required for NADPH oxidase activity in PMNs or macrophages or for endotoxin-triggered tumor necrosis factor-alpha release and signal transduction BMDMs.
Publisher:American Society of Hematology (U.S.A.)
Item Type:Article

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