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Scara5 is a ferritin receptor mediating non-transferrin iron delivery

Item Type:Article
Title:Scara5 is a ferritin receptor mediating non-transferrin iron delivery
Creators Name:Li, J.Y. and Paragas, N. and Ned, R.M. and Qiu, A. and Viltard, M. and Leete, T. and Drexler, I.R. and Chen, X. and Sanna-Cherchi, S. and Mohammed, F. and Williams, D. and Lin, C.S. and Schmidt-Ott, K.M. and Andrews, N.C. and Barasch, J.
Abstract:Developing organs require iron for a myriad of functions, but embryos deleted of the major adult transport proteins, transferrin or its receptor transferrin receptor1 (TfR1(-/-)), still initiate organogenesis, suggesting that non-transferrin pathways are important. To examine these pathways, we developed chimeras composed of fluorescence-tagged TfR1(-/-) cells and untagged wild-type cells. In the kidney, TfR1(-/-) cells populated capsule and stroma, mesenchyme and nephron, but were underrepresented in ureteric bud tips. Consistently, TfR1 provided transferrin to the ureteric bud, but not to the capsule or the stroma. Instead of transferrin, we found that the capsule internalized ferritin. Since the capsule expressed a novel receptor called Scara5, we tested its role in ferritin uptake and found that Scara5 bound serum ferritin and then stimulated its endocytosis from the cell surface with consequent iron delivery. These data implicate cell type-specific mechanisms of iron traffic in organogenesis, which alternatively utilize transferrin or non-transferrin iron delivery pathways.
Keywords:DEVBIO, Biological Transport, Cell Line, Chimera, Endocytosis, Ferritins, Iron, Iron-Binding Proteins, Kidney, Inbred C57BL Mice, Knockout Mice, Morphogenesis, Cell Surface Receptors, Transferrin Receptors, Class A Scavenger Receptors, Transferrin, Animals, Mice
Source:Developmental Cell
Publisher:Cell Press
Page Range:35-46
Date:20 January 2009
Official Publication:https://doi.org/10.1016/j.devcel.2008.12.002
PubMed:View item in PubMed

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