Helmholtz Gemeinschaft


CCR7-deficient mice develop atypically persistent germinal centers in response to thymus-independent type 2 antigens

Item Type:Article
Title:CCR7-deficient mice develop atypically persistent germinal centers in response to thymus-independent type 2 antigens
Creators Name:Achtman, A.H. and Hoepken, U.E. and Bernert, C. and Lipp, M.
Abstract:Thymus-independent type 2 (TI-2) antigens are repetitive antigens capable of eliciting antibody responses without T cell help. They are important in the immune response against encapsulated bacteria and as a rapid first line of defense against pathogens. TI-2 antigens induce strong proliferation in extrafollicular foci. However, any germinal centers forming in response to TI-2 antigens involute synchronously 5 days after immunization. This is thought to be caused by the lack of T cell help. Surprisingly, immunization of mice deficient for the homeostatic chemokine receptor CCR7 with TI-2 antigens resulted not only in the expected, vigorous extrafollicular plasma cell response but also in persisting splenic germinal centers. This was observed for two different TI-2 antigens, heat-killed Streptococcus pneumoniae and (4-hydroxy-3-nitrophenyl)acetyl-Ficoll. Germinal centers induced by TI-2 and thymus-dependent (TD) antigens were located in the periarteriolar area of the white pulp in CCR7 knockout mice, corresponding to the T zone of wild-type (WT) mice. The TI-2-induced germinal centers contained peripheral rings of follicular dendritic cells and unusually for TI-2-induced germinal centers, T cells. The licensing responsible for their atypical persistence did not endow TI-2-induced germinal centers with the full range of characteristics of classic germinal centers induced by TD antigens. Thus, class-switching, affinity maturation, and memory B cell generation were not increased in CCR7-deficient mice. It seems unlikely that a defect in regulatory T cell (Treg) location was responsible for the atypical persistence of TI-2-induced germinal centers, as Tregs were comparably distributed in germinal centers of CCR7-deficient and WT mice.
Keywords:Homeostatic Chemokine, Spleen, Antibody, TI-2, NP-Ficoll, Animals, Mice
Source:Journal of Leukocyte Biology
Publisher:Federation of American Societies for Experimental Biology (U.S.A.)
Page Range:409-417
Date:March 2009
Official Publication:https://doi.org/10.1189/jlb.0308162
PubMed:View item in PubMed

Repository Staff Only: item control page

Open Access
MDC Library