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Global analysis of cellular protein translation by pulsed SILAC

Item Type:Article
Title:Global analysis of cellular protein translation by pulsed SILAC
Creators Name:Schwanhaeusser, B. and Gossen, M. and Dittmar, G. and Selbach, M.
Abstract:Current methods for system-wide gene expression analysis detect changes in mRNA abundance, but neglect regulation at the level of translation. Pulse labeling with stable isotopes has been used to measure protein turnover rates, but this does not directly provide information about translation rates. Here, we developed pulsed stable isotope labeling by amino acids in cell culture (pSILAC) with two heavy isotope labels to directly quantify protein translation on a proteome-wide scale. We applied the method to cellular iron homeostasis as a model system and demonstrate that it can confidently identify proteins that are translationally regulated by iron availability.
Keywords:Iron Homeostasis, SILAC, Translation Regulation
Source:Proteomics
ISSN:1615-9853
Publisher:Wiley (Germany)
Volume:9
Number:1
Page Range:205-209
Date:January 2009
Official Publication:https://doi.org/10.1002/pmic.200800275
PubMed:View item in PubMed

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