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Aberrant methylation of human L- and M-fructose 1,6-bisphosphatase genes in cancer

Item Type:Article
Title:Aberrant methylation of human L- and M-fructose 1,6-bisphosphatase genes in cancer
Creators Name:Bigl, M. and Jandrig, B. and Horn, L.C. and Eschrich, K.
Abstract:A possible epigenetic regulation of the two isoenzymes of fructose 1,6-bisphosphatase (FBPase) was studied in liver, muscle, mamma, breast cancer and in different cancer cell lines. Results obtained after bisulfite sequencing revealed a different CpG methylation of both promoters in liver, muscle and breast tissue which is putatively involved in the cell-type specific gene expression of the two enzymes. In tumor cell lines, demethylation with 5-aza-deoxycytidine activated the expression of both isoenzymes. Additional inhibition of histone deacetylase with trichostatin A further increased FBPase mRNA concentrations. Since cancers typically have an abnormal energy metabolism and exhibit a low gluconeogenic phenotype, it was studied whether promoter methylation contributes to the decreased expression of FBPase in breast cancer. When non-malignant and malignant tissue samples from the same patient were compared a correlation between an increase of FBPase promoter methylation and a decrease of FBPase mRNA levels was observed.
Keywords:Breast Cancer, Cell Line, DNA Methylation, Fructose 1,6-Bisphosphatase, Gene Expression, Gluconeogenesis, Promoter, Tissue-Specific Expression
Source:Biochemical and Biophysical Research Communications
Publisher:Academic Press
Page Range:720-724
Date:12 December 2008
Official Publication:https://doi.org/10.1016/j.bbrc.2008.10.045
PubMed:View item in PubMed

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