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Regulation of neurocoel morphogenesis by Pard6 gamma b

Item Type:Article
Title:Regulation of neurocoel morphogenesis by Pard6 gamma b
Creators Name:Munson, C. and Huisken, J. and Bit-Avragim, N. and Kuo, T. and Dong, P.D. and Ober, E.A. and Verkade, H. and Abdelilah-Seyfried, S. and Stainier, D.Y.
Abstract:The Par3/Par6/aPKC protein complex plays a key role in the establishment and maintenance of apicobasal polarity, a cellular characteristic essential for tissue and organ morphogenesis, differentiation and homeostasis. During a forward genetic screen for liver and pancreas mutants, we identified a pard6gammab mutant, representing the first known pard6 mutant in a vertebrate organism. pard6gammab mutants exhibit defects in epithelial tissue development as well as multiple lumens in the neural tube. Analyses of the cells lining the neural tube cavity, or neurocoel, in wildtype and pard6gammab mutant embryos show that lack of Pard6gammab function leads to defects in mitotic spindle orientation during neurulation. We also found that the PB1 (aPKC-binding) and CRIB (Cdc-42-binding) domains and the KPLG amino acid sequence within the PDZ domain (Pals1-and Crumbs binding) are not required for Pard6gammab localization but are essential for its function in neurocoel morphogenesis. Apical membranes are reduced, but not completely absent, in mutants lacking the zygotic, or both the maternal and zygotic, function of pard6gammab, leading us to examine the localization and function of the three additional zebrafish Pard6 proteins. We found that Pard6alpha, but not Pard6beta or Pard6gammaa, could partially rescue the pard6gammab(s441) mutant phenotypes. Altogether, these data indicate a previously unappreciated functional diversity and complexity within the vertebrate pard6 gene family.
Keywords:Par6, Neurulation, Apicobasal Polarity, Epithelium, Signal Transducing Adaptor Proteins, Cell Polarity, Epithelium, Mitotic Spindle Apparatus, Morphogenesis, Mutation, Neural Tube, Neurulation, Zebrafish Proteins, Animals, Zebrafish
Source:Developmental Biology
Publisher:Academic Press (U.S.A.)
Page Range:41-54
Date:1 December 2008
Official Publication:https://doi.org/10.1016/j.ydbio.2008.08.033
PubMed:View item in PubMed

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