Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

Cargo-selected transport from the mitochondria to peroxisomes is mediated by vesicular carriers

Official URL:https://doi.org/10.1016/j.cub.2007.12.038
PubMed:View item in PubMed
Creators Name:Neuspiel, M. and Schauss, A.C. and Braschi, E. and Zunino, R. and Rippstein, P. and Rachubinski, R.A. and Andrade-Navarro, M.A. and McBride, H.M.
Journal Title:Current Biology
Journal Abbreviation:Curr Biol
Volume:18
Number:2
Page Range:102-108
Date:22 January 2008
Keywords:Cellbio, Proteins, Animals, Monkeys
Abstract:Mitochondria and peroxisomes share a number of common biochemical processes, including the beta oxidation of fatty acids and the scavenging of peroxides. Here, we identify a new outer-membrane mitochondria-anchored protein ligase (MAPL) containing a really interesting new gene (RING)-finger domain. Overexpression of MAPL leads to mitochondrial fragmentation, indicating a regulatory function controlling mitochondrial morphology. In addition, confocal- and electron-microscopy studies of MAPL-YFP led to the observation that MAPL is also incorporated within unique, DRP1-independent, 70-100 nm diameter mitochondria-derived vesicles (MDVs). Importantly, vesicles containing MAPL exclude another outer-membrane marker, TOM20, and vesicles containing TOM20 exclude MAPL, indicating that MDVs selectively incorporate their cargo. We further demonstrate that MAPL-containing vesicles fuse with a subset of peroxisomes, marking the first evidence for a direct relationship between these two functionally related organelles. In contrast, a distinct vesicle population labeled with TOM20 does not fuse with peroxisomes, indicating that the incorporation of specific cargo is a primary determinant of MDV fate. These data are the first to identify MAPL, describe and characterize MDVs, and define a new intracellular transport route between mitochondria and peroxisomes.
ISSN:0960-9822
Publisher:Cell Press (U.S.A.)
Item Type:Article

Repository Staff Only: item control page

Open Access
MDC Library