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Titin, myosin light chains, and c-protein in the developing and failing human heart

Item Type:Article
Title:Titin, myosin light chains, and c-protein in the developing and failing human heart
Creators Name:Morano, I. and Haedicke, K. and Grom, S. and Koch, A. and Schwinger, R.H.G. and Boehm, M. and Bartel, S. and Erdmann, E. and Krause, E.G.
Abstract:We investigated relative amounts of titin, myosin light chains (MLC), C-protein, and myosin heavy chains (MHC) in the functionally intact contractile apparatus of embryonic, adult normal, and adult failing human left ventricle by SDS polyacrylamide gel electrophoresis and Western blot analysis. The amount of titin and the titin-MHC ratio was significantly (P < 0.05) lower in the embryonic and adult failing human compared to the adult normal fibres. Additionally, we found a protein band having a lower molecular weight but the same immunoreactivity as native titin (fast-migrating titin; FM-titin) in the failing heart only. The MLC-1/MLC-2 ratio was about 1.0 in normal and failing hearts but about 2.0 in the embryonic heart. Relative amount of C-protein was the same in normal and failing fibres but lower in the embryonic ventricles. Length-tension ratio of chemically skinned fibres prepared from terminally failing hearts was impaired compared to normal heart fibres. We conclude that both the reduced titin/MHC ratio and the expression of a structurally different titin form may be involved in the impaired contractile function of the terminally failing human heart.
Keywords:Titin, C-Protein, Myosin, Myosin-Light Chains, Human Heart
Source:Journal of Molecular and Cellular Cardiology
ISSN:0022-2828
Publisher:Elsevier (The Netherlands)
Volume:26
Number:3
Page Range:361-368
Date:1 March 1994
Official Publication:https://doi.org/10.1006/jmcc.1994.1045
PubMed:View item in PubMed

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