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Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy

Item Type:Article
Title:Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy
Creators Name:Schermelleh, L. and Carlton, P.M. and Haase, S. and Shao, L. and Winoto, L. and Kner, P. and Burke, B. and Cardoso, M.C. and Agard, D.A. and Gustafsson, M.G. and Leonhardt, H. and Sedat, J.W.
Abstract:Fluorescence light microscopy allows multicolor visualization of cellular components with high specificity, but its utility has until recently been constrained by the intrinsic limit of spatial resolution. We applied three-dimensional structured illumination microscopy (3D-SIM) to circumvent this limit and to study the mammalian nucleus. By simultaneously imaging chromatin, nuclear lamina, and the nuclear pore complex (NPC), we observed several features that escape detection by conventional microscopy. We could resolve single NPCs that colocalized with channels in the lamin network and peripheral heterochromatin. We could differentially localize distinct NPC components and detect double-layered invaginations of the nuclear envelope in prophase as previously seen only by electron microscopy. Multicolor 3D-SIM opens new and facile possibilities to analyze subcellular structures beyond the diffraction limit of the emitted light.
Keywords:Cell Line, Cell Nucleus, Chromatin, Fluorescent Dyes, Heterochromatin, Three-Dimensional Imaging, Indoles, Interphase, Lamins, Confocal Microscopy, Fluorescence Microscopy, Myoblasts, Nuclear Envelope, Nuclear Lamina, Nuclear Pore, Optics and Photonics, Animals, Mice
Publisher:American Association for the Advancement of Science
Page Range:1332-1336
Date:6 June 2008
Official Publication:https://doi.org/10.1126/science.1156947
PubMed:View item in PubMed

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