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Differential recognition of alpha1-antitrypsin-elastase and alpha1-antichymotrypsin-cathepsin G complexes by the low density lipoprotein receptor-related protein

Item Type:Article
Title:Differential recognition of alpha1-antitrypsin-elastase and alpha1-antichymotrypsin-cathepsin G complexes by the low density lipoprotein receptor-related protein
Creators Name:Poller, W. and Willnow, T.E. and Hilpert, J. and Herz, J.
Abstract:Two multifunctional receptors, low density lipoprotein receptor-related protein (LRP) and gp330, have been implicated in the cellular uptake and degradation of a wide spectrum of functionally diverse ligands including plasma lipoproteins, proteases, and proteinase-inhibitor complexes. The two receptors show distinct tissue-specific expression patterns, suggesting different physiological functions. We have examined the cellular degradation of two serine proteinase inhibitor (serpin)-protease complexes, alpha 1-antitrypsin-neutrophil elastase (alpha 1AT.NEL) and alpha 1-antichymotrypsin-cathepsin G (alpha 1ACT.CathG) by normal murine fibroblasts (MEF) expressing LRP, and by a mutant fibroblast cell line (PEA13) which is genetically deficient for LRP. alpha 1AT.NEL complexes bound to LRP on ligand blots and were degraded efficiently by the MEF cells, but not by PEA13 cells. Degradation of the complexes was also significantly reduced by antibodies directed against LRP, further suggesting that fibroblasts require LRP for the cellular uptake and degradation of alpha 1AT.NEL complexes. In contrast to alpha 1AT.NEL, MEF cells did not degrade alpha 1ACT.CathG complexes. However, these complexes were rapidly degraded by the rat embryonal carcinoma cell line L2p58 which abundantly expresses gp330, raising the possibility that the alpha 1ACT.CathG complex might be recognized by gp330. Both complexes were efficiently metabolized by the hepatoma cell line HepG2, presumably involving the serpin-enzyme complex receptor. The differential recognition of serpin-protease complexes by fibroblasts and hepatoma cells, however, indicates that LRP, gp330, and the serpin-enzyme complex receptor are distinct proteins.
Keywords:alpha 1-Antichymotrypsin, alpha 1-Antitrypsin, Cathepsin G, Cell Line, Immunologic Receptors, LDL-Receptor Related Protein 1, Leukocyte Elastase, Pancreatic Elastase, Protein Binding, LDL Receptors, Serine Endopeptidases, Animals, Mice
Source:Journal of Biological Chemistry
ISSN:0021-9258
Publisher:American Society for Biochemistry and Molecular Biology (U.S.A.)
Volume:270
Number:6
Page Range:2841-2845
Date:10 February 1995
Official Publication:https://doi.org/10.1074/jbc.270.6.2841
PubMed:View item in PubMed

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