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Nuclear localization of the zebrafish tight junction protein nagie oko

Item Type:Article
Title:Nuclear localization of the zebrafish tight junction protein nagie oko
Creators Name:Bit-Avragim, N. and Rohr, S. and Rudolph, F. and Van der Ven, P. and Furst, D. and Eichhorst, J. and Wiesner, B. and Abdelilah-Seyfried, S.
Abstract:The tight junctions-associated MAGUK protein nagie oko is closely related to Drosophila Stardust, mouse protein associated with lin-seven 1 (Pals1), and human MAGUK p55 subfamily member 5 (Mpp5). As a component of the evolutionarily conserved Crumbs protein complex, nagie oko is essential for the maintenance of epithelial cell polarity. Here, we show that nagie oko contains a predicted nuclear export and two conserved nuclear localization signals. We find that loss of the predicted nuclear export signal results in nuclear protein accumulation. We show that nagie oko nuclear import is redundantly controlled by the two nuclear localization signals and the evolutionarily conserved region 1 (ECR1), which links nagie oko with Par6-aPKC. Finally, deletion forms of nagie oko that lack nuclear import and export signals complement several nagie oko mutant defects in cell polarity and epithelial integrity. This finding provides an entry point to potentially novel and unknown roles of this important cell polarity regulator.
Keywords:Nagie Oko, Nuclear Import, Nuclear Export, Cell Polarity, aPKC, Mpp5, Pals1, Animals, Zebrafish
Source:Developmental Dynamics
ISSN:1058-8388
Publisher:Wiley (U.S.A.)
Volume:237
Number:1
Page Range:83-90
Date:January 2008
Official Publication:https://doi.org/10.1002/dvdy.21389
PubMed:View item in PubMed

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