Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

Protein interaction screening by quantitative immunoprecipitation combined with knockdown (QUICK)

Item Type:Article
Title:Protein interaction screening by quantitative immunoprecipitation combined with knockdown (QUICK)
Creators Name:Selbach, M. and Mann, M.
Abstract:Present screening methods for protein-protein interactions (PPIs) rely on the overexpression of artificial fusion proteins, making it difficult to assess in vivo relevance. Here we combine stable isotope labeling with amino acids in cell culture (SILAC), RNA interference (RNAi), coimmunoprecipitation and quantitative mass-spectrometry analysis to detect cellular interaction partners of endogenous proteins in mammalian cells with very high confidence. We used this screen to identify interaction partners of beta-catenin and Cbl.
Keywords:Gene Silencing, Immunoassay, Immunoprecipitation, Mass Spectrometry, Protein Interaction Mapping
Source:Nature Methods
ISSN:1548-7091
Publisher:Nature Publishing Group (U.S.A.)
Volume:3
Number:12
Page Range:981-983
Date:December 2006
Official Publication:https://doi.org/10.1038/nmeth972
PubMed:View item in PubMed

Repository Staff Only: item control page

Open Access
MDC Library