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Survival of resting mature B lymphocytes depends on BCR signaling via the Igalpha/beta heterodimer

Item Type:Article
Title:Survival of resting mature B lymphocytes depends on BCR signaling via the Igalpha/beta heterodimer
Creators Name:Kraus, M. and Alimzhanov, M.B. and Rajewsky, N. and Rajewsky, K.
Abstract:We previously showed that type I interferon-induced, Cre-mediated ablation of surface BCR expression in mature B cells through Ig-heavy chain deletion results in apoptosis of these cells. This led to the hypothesis that survival signals from the BCR are vital for mature B cells. Here, we test two critical assumptions of this model. First, we demonstrate loss of mature B cells upon induced mutation of a signaling module of the BCR, not precluding BCR surface expression. Second, we show that the cells are also lost upon BCR inactivation in the absence of an exogenous inducer like interferon, excluding that cell death depends on previous cellular activation by the latter. Kinetic data demonstrate that BCR-less mature B cells have a severely reduced lifespan, with a half-life of 3-6 days. Together these results establish that BCR signaling is required to keep resting mature B cells alive in vivo.
Keywords:CD Antigens, CD79 Antigens, B-Lymphocytes, Cell Death, Cell Differentiation, Cell Membrane, Cell Survival, Dimerization, Interferons, Inbred C57BL Mice, Transgenic Mice, Mutation, B-Cell Antigen Receptors, Signal Transduction, Animals, Mice
Source:Cell
ISSN:0092-8674
Publisher:Cell Press (U.S.A.)
Volume:117
Number:6
Page Range:787-800
Date:11 June 2004
Official Publication:https://doi.org/10.1016/j.cell.2004.05.014
PubMed:View item in PubMed

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