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Frequency of blood CX3CR1-positive natural killer cells correlates with disease activity in multiple sclerosis patients

Item Type:Article
Title:Frequency of blood CX3CR1-positive natural killer cells correlates with disease activity in multiple sclerosis patients
Creators Name:Infante-Duarte, C. and Weber, A. and Kratzschmar, J. and Prozorovski, T. and Pikol, S. and Hamann, I. and Bellmann-Strobl, J. and Aktas, O. and Doerr, J. and Wuerfel, J. and Stuerzebecher, C.S. and Zipp, F.
Abstract:Multiple sclerosis (MS) is an autoimmune disease of the central nervous system (CNS) characterized by enormous variability in its clinical presentation and course, and for which clear diagnostic parameters are lacking. Here we performed an RNA screen in peripheral mononuclear cells from relapsing-remitting (RR) and primary progressive (PP) MS patients compared with healthy donors (HD) that indicated, among other findings, a role for the chemokine receptor CX3CR1 as a diagnostic marker. Gene expression and flow cytometric analyses demonstrated a significantly lower expression of CX3CR1 in MS patients compared with healthy individuals. The subpopulation of cells responsible for causing this reduced expression of CX3CR1 consisted exclusively of natural killer (NK) cells. Importantly, we found a correlation between disease activity and frequency of CX3CR1-positive NK cells in RRMS patients. These findings emphasize the role of NK cells in the development and course of MS and provide evidence for CX3CR1 expression as a marker for MS patients and disease activity.
Keywords:Monoclonal Antibodies, Biotinylation, CD8-Positive T-Lymphocytes, Cell Survival, Down-Regulation, Flow Cytometry, Gene Expression Regulation, Natural Killer Cells, Membrane Proteins, Relapsing-Remitting Multiple Sclerosis, Oligonucleotide Array Sequence Analysis, Chemokine Receptors, Recurrence, Remission Induction, Reverse Transcriptase Polymerase Chain Reaction
Source:FASEB Journal
Publisher:Federation of American Societies for Experimental Biology
Page Range:1902-1904
Date:November 2005
Official Publication:https://doi.org/10.1096/fj.05-3832fje
PubMed:View item in PubMed

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