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Protein production and purification

Item Type:Review
Title:Protein production and purification
Creators Name:Gräslund, S. and Nordlund, P. and Weigelt, J. and Hallberg, M. and Bray, J. and Gileadi, O. and Knapp, S. and Oppermann, U. and Arrowsmith, C. and Hui, R. and Ming, J. and dhe-Paganon, S. and Park, H.W. and Savchenko, A. and Yee, A. and Edwards, A. and Vincentelli, R. and Cambillau, C. and Kim, R. and Kim, S.H. and Rao, Z. and Shi, Y. and Terwilliger, T.C. and Kim, C.Y. and Hung, L.W. and Waldo, G.S. and Peleg, Y. and Albeck, S. and Unger, T. and Dym, O. and Prilusky, J. and Sussman, J.L. and Stevens, R.C. and Lesley, S.A. and Wilson, I.A. and Joachimiak, A. and Collart, F. and Dementieva, I. and Donnelly, M.I. and Eschenfeldt, W.H. and Kim, Y.M. and Stols, L. and Wu, R. and Zhou, M. and Burley, S.K. and Emtage, J.S. and Sauder, J.M. and Thompson, D. and Bain, K. and Luz, J. and Gheyi, T. and Zhang, F. and Atwell, S. and Almo, S.C. and Bonanno, J.B. and Fiser, A. and Swaminathan, S. and Studier, F.W. and Chance, M.R. and Sali, A. and Acton, T.B. and Xiao, R. and Zhao, L. and Ma, L.C. and Hunt, J.F. and Tong, L. and Cunningham, K. and Inouye, M. and Anderson, S. and Janjua, H. and Shastry, R. and Ho, C.K. and Wang, D. and Wang, H. and Jiang, M. and Montelione, G.T. and Stuart, D.I. and Owens, R.J. and Daenke, S. and Schütz, A. and Heinemann, U. and Yokoyama, S. and Büssow, K. and Gunsalus, K.C.
Abstract:In selecting a method to produce a recombinant protein, a researcher is faced with a bewildering array of choices as to where to start. To facilitate decision-making, we describe a consensus 'what to try first' strategy based on our collective analysis of the expression and purification of over 10,000 different proteins. This review presents methods that could be applied at the outset of any project, a prioritized list of alternate strategies and a list of pitfalls that trip many new investigators.
Keywords:Chemical Fractionation, Physical Chemistry, Protein Engineering, Proteomics, Recombinant Proteins
Source:Nature Methods
Publisher:Nature Publishing Group
Page Range:135-146
Date:February 2008
Official Publication:https://doi.org/10.1038/nmeth.f.202
PubMed:View item in PubMed

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