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Identification of a negative regulatory domain in the human papillomavirus type 18 promoter: interaction with the transcriptional repressor YY1

Item Type:Article
Title:Identification of a negative regulatory domain in the human papillomavirus type 18 promoter: interaction with the transcriptional repressor YY1
Creators Name:Bauknecht, T. and Angel, P. and Royer, H.D. and zur Hausen, H.
Abstract:The human papillomavirus type 18 (HPV-18) promoter contains a TPA responsive element (TRE) which confers TPA responsiveness on a heterologous promoter. In the context of the HPV-18 promoter, however, this AP-1 site is inactive. We have identified a negative regulatory domain in the HPV-18 promoter which represses the constitutive and TPA-induced AP-1 activity. This negative regulatory sequence has been mapped to 44 nucleotides (OL13). We identified this element as a transcriptional silencer based on its ability to interfere with transcriptional initiation. This HPV-18 silencer domain was narrowed down further to 23 nucleotides, the OL13B element, which bears similarity to three other silencer sequences, present in the mouse N-ras gene upstream regulatory region, the mouse albumin gene enhancer and the adeno-associated virus P5 promoter. The transcriptional repressor protein YY1, which negatively regulates the P5 promoter, binds to the HPV-18 silencer with high affinity. Mutation of the YY1 binding site leads to an enhanced activity of the HPV-18 promoter, strongly suggesting that YY1 plays an important role in controlling HPV-18 early gene expression.
Keywords:Base Sequence, Binding Sites, Cultured Cells, Chloramphenicol O-Acetyltransferase, DNA, DNA-Binding Proteins, Genetic Enhancer Elements, Erythroid-Specific DNA-Binding Factors, Viral Genes, Hela Cells, Molecular Sequence Data, Mutation, Papillomaviridae, Polymerase Chain Reaction, Genetic Promoter Regions, Proto-Oncogene Proteins c-jun, Tetradecanoylphorbol Acetate, Transcription Factors, Genetic Transcription, YY1 Transcription Factor
Source:EMBO Journal
ISSN:0261-4189
Publisher:Nature Publishing Group
Volume:11
Number:12
Page Range:4607-4617
Date:December 1992
Official Publication:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC557037
PubMed:View item in PubMed

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