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Long-term, multilineage hematopoiesis occurs in the combined absence of beta-catenin and gamma-catenin

Official URL:https://doi.org/10.1182/blood-2007-07-102558
PubMed:View item in PubMed
Creators Name:Jeannet, G. and Scheller, M. and Scarpellino, L. and Duboux, S. and Gardiol, N. and Back, J. and Kuttler, F. and Malanchi, I. and Birchmeier, W. and Leutz, A. and Huelsken, J. and Held, W.
Journal Title:Blood
Journal Abbreviation:Blood
Page Range:142-149
Date:1 January 2008
Keywords:Bone Marrow Cells, Cell Lineage, Hematopoiesis, Hematopoietic Stem Cells, Hybridomas, Signal Transduction, Spleen, T-Lymphocytes, Thymus Gland, Wnt Proteins, beta Catenin, gamma Catenin, Animals, Mice
Abstract:The canonical Wnt signaling pathway plays key roles in stem cell maintenance, progenitor cell expansion and lineage decisions. Transcriptional responses induced by Wnt depend on the association of either beta-catenin or gamma-catenin with TCF/LEF transcription factors. Here we show that hematopoiesis, including thymopoiesis, is normal in the combined absence of beta- and gamma-catenin. Double-deficient hematopoietic stem cells (HSCs) maintain long-term repopulation capacity and multi lineage differentiation potential. Unexpectedly, two independent ex vivo reporter gene assays show that Wnt signal transmission is maintained in double-deficient HSCs, thymocytes, or peripheral T cells. In contrast, Wnt signaling is strongly reduced in thymocytes lacking TCF-1 or in non-hematopoietic cells devoid of beta-catenin. These data provide the first evidence that hematopoietic cells can transduce canonical Wnt signals in the combined absence of beta- and gamma-catenin.
Publisher:American Society of Hematology (U.S.A.)
Item Type:Article

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