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MiR-150 controls B cell differentiation by targeting the transcription factor c-Myb

Item Type:Article
Title:MiR-150 controls B cell differentiation by targeting the transcription factor c-Myb
Creators Name:Xiao, C. and Calado, D.P. and Galler, G. and Thai, T.H. and Patterson, H.C. and Wang, J. and Rajewsky, N. and Bender, T.P. and Rajewsky, K.
Abstract:MiR-150 is a microRNA (miRNA) specifically expressed in mature lymphocytes, but not their progenitors. A top predicted target of miR-150 is c-Myb, a transcription factor controlling multiple steps of lymphocyte development. Combining loss- and gain-of-function gene targeting approaches for miR-150 with conditional and partial ablation of c-Myb, we show that miR-150 indeed controls c-Myb expression in vivo in a dose-dependent manner over a narrow range of miRNA and c-Myb concentrations and that this dramatically affects lymphocyte development and response. Our results identify a key transcription factor as a critical target of a stage-specifically expressed miRNA in lymphocytes and suggest that this and perhaps other miRNAs have evolved to control the expression of just a few critical target proteins in particular cellular contexts.
Keywords:MOLIMMUNO, RNA, Animals, Mice
Publisher:Cell Press
Page Range:146-159
Date:5 October 2007
Additional Information:Erratum in: Cell 165(4): 1027.
Official Publication:https://doi.org/10.1016/j.cell.2007.07.021
PubMed:View item in PubMed

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