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Modulation of muscle contraction by a cell-permeable peptide

Item Type:Article
Title:Modulation of muscle contraction by a cell-permeable peptide
Creators Name:Tünnemann, G. and Karczewski, P. and Haase, H. and Cardoso, M.C. and Morano, I.
Abstract:In contrast to immortal cell lines, primary cells are hardly susceptible to intracellular delivery methods such as transfection. In this study, we evaluated the direct delivery of several cell-permeable peptides under noninvasive conditions into living primary adult rat cardiomyocytes. We specifically monitored the functional effects of a cell-permeable peptide containing the 15 amino acid N-terminal peptide from human ventricular light chain-1 (VLC-1) on contraction and intracellular Ca2+ signals after electrical stimulation in primary adult cardiomyocytes. The transducible VLC-1 variant was taken up by cardiomyocytes within 5 min with more than 95% efficiency and localized to sarcomeric structures. Analysis of the functional effects of the cell-permeable VLC-1 revealed an enhancement of the intrinsic contractility of cardiomyocytes without affecting the intracellular Ca2+. Therefore, peptide transduction mediated by cell-penetrating peptides represents not only a unique strategy to enhance heart muscle function with no secondary effect on intracellular Ca2+ but also an invaluable tool for the modulation and manipulation of protein interactions in general and in primary cells.
Keywords:Ventricular Light Chain-1 (VLC-1), Cell Penetrating Peptide (CPP), Peptide Transduction, Cardiomyocytes, Drug Delivery, Animals, Rats
Source:Journal of Molecular Medicine
Page Range:1405-1412
Date:December 2007
Additional Information:The original publication is available at www.springerlink.com
Official Publication:https://doi.org/10.1007/s00109-007-0238-6
PubMed:View item in PubMed

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