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| Item Type: | Article |
|---|---|
| Title: | Modulation of muscle contraction by a cell-permeable peptide |
| Creators Name: | Tünnemann, G., Karczewski, P., Haase, H., Cardoso, M.C. and Morano, I. |
| Abstract: | In contrast to immortal cell lines, primary cells are hardly susceptible to intracellular delivery methods such as transfection. In this study, we evaluated the direct delivery of several cell-permeable peptides under noninvasive conditions into living primary adult rat cardiomyocytes. We specifically monitored the functional effects of a cell-permeable peptide containing the 15 amino acid N-terminal peptide from human ventricular light chain-1 (VLC-1) on contraction and intracellular Ca2+ signals after electrical stimulation in primary adult cardiomyocytes. The transducible VLC-1 variant was taken up by cardiomyocytes within 5 min with more than 95% efficiency and localized to sarcomeric structures. Analysis of the functional effects of the cell-permeable VLC-1 revealed an enhancement of the intrinsic contractility of cardiomyocytes without affecting the intracellular Ca2+. Therefore, peptide transduction mediated by cell-penetrating peptides represents not only a unique strategy to enhance heart muscle function with no secondary effect on intracellular Ca2+ but also an invaluable tool for the modulation and manipulation of protein interactions in general and in primary cells. |
| Keywords: | Ventricular Light Chain-1 (VLC-1), Cell Penetrating Peptide (CPP), Peptide Transduction, Cardiomyocytes, Drug Delivery, Animals, Rats |
| Source: | Journal of Molecular Medicine |
| ISSN: | 0946-2716 |
| Publisher: | Springer |
| Volume: | 85 |
| Number: | 12 |
| Page Range: | 1405-1412 |
| Date: | December 2007 |
| Additional Information: | The original publication is available at www.springerlink.com |
| Official Publication: | https://doi.org/10.1007/s00109-007-0238-6 |
| PubMed: | View item in PubMed |
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