Helmholtz Gemeinschaft


MeCP2 interacts with HP1 and modulates its heterochromatin association during myogenic differentiation

[img] PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader

Item Type:Article
Title:MeCP2 interacts with HP1 and modulates its heterochromatin association during myogenic differentiation
Creators Name:Agarwal, N. and Hardt, T. and Brero, A. and Nowak, D. and Rothbauer, U. and Becker, A. and Leonhardt, H. and Cardoso, M.C.
Abstract:There is increasing evidence of crosstalk between epigenetic modifications such as histone and DNA methylation, recognized by HP1 and methyl CpG-binding proteins, respectively. We have previously shown that the level of methyl CpG-binding proteins increased dramatically during myogenesis leading to large-scale heterochromatin reorganization. In this work, we show that the level of HP1 isoforms did not change significantly throughout myogenic differentiation but their localization did. In particular, HP1 relocalization to heterochromatin correlated with MeCP2 presence. Using co-immunoprecipitation assays, we found that these heterochromatic factors interact in vivo via the chromo shadow domain of HP1 and the first 55 amino acids of MeCP2. We propose that this dynamic interaction of HP1 and MeCP2 increases their concentration at heterochromatin linking two major gene silencing pathways to stabilize transcriptional repression during differentiation.
Keywords:Binding Sites, Cell Differentiation, Cell Line, Non-Histone Chromosomal Proteins, Heterochromatin, Methyl-CpG-Binding Protein 2, Muscle Development, Myoblasts, Protein Isoforms, Tertiary Protein Structure, Animals, Mice, Rats
Source:Nucleic Acids Research
Publisher:Oxford University Press
Page Range:5402-5408
Date:August 2007
Official Publication:https://doi.org/10.1093/nar/gkm599
PubMed:View item in PubMed

Repository Staff Only: item control page


Downloads per month over past year

Open Access
MDC Library