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Selective targeting of adenoviral vectors to neural precursor cells in the hippocampus of adult mice: new prospects for in situ gene therapy

Item Type:Article
Title:Selective targeting of adenoviral vectors to neural precursor cells in the hippocampus of adult mice: new prospects for in situ gene therapy
Creators Name:Schmidt, A. and Haas, S.J. P. and Hildebrandt, S. and Scheibe, J. and Eckhoff, B. and Racek, T. and Kempermann, G. and Wree, A. and Putzer, B.M.
Abstract:The adult brain contains neural precursor cells (NPC) that are attracted to brain lesions, such as areas of neurodegeneration, ischemia and cancer. This suggests that NPC engineered to promote lineage-specific differentiation or to express therapeutic genes might become a valuable tool for restorative cell therapy and for targeting therapeutic genes to diseased brain regions. Here we report the identification of NPC specific ligands from phage display peptide libraries and show their potential to selectively direct adenovirus mediated gene transfer to NPC in adult mice. Identified peptides mediated specific virus binding and internalization to cultured neurospheres. Importantly, peptide-mediated adenoviral vector infection was restricted to precursor cells in the hippocampal dentate gyrus of pNestin-GFP transgenic or C57BL/6 mice. Our approach represents a novel way for specific manipulation of NPC in the adult brain and may have major implications for the use of precursor cells as therapeutic delivery vehicles in the central nervous system.
Keywords:Neural Precursor/Stem Cells, Gene Therapy, Dentate Gyrus, Adenoviral Vectors, Phage Display, Animals, Mice
Source:Stem Cells
ISSN:1066-5099
Publisher:Karger (Switzerland)
Volume:25
Number:11
Page Range:2910-2918
Date:November 2007
Official Publication:https://doi.org/10.1634/stemcells.2007-0238
PubMed:View item in PubMed

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