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Global transcriptome analysis of murine embryonic stem cell-derived cardiomyocytes

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Item Type:Article
Title:Global transcriptome analysis of murine embryonic stem cell-derived cardiomyocytes
Creators Name:Doss, M.X. and Winkler, J. and Chen, S. and Hippler-Altenburg, R. and Sotiriadou, I. and Halbach, M. and Pfannkuche, K. and Liang, H. and Schulz, H. and Hummel, O. and Huebner, N. and Rottscheidt, R. and Hescheler, J. and Sachinidis, A.
Abstract:BACKGROUND: Characterisation of gene expression signatures for cardiomyocytes derived from embryonic stem (ES) cells will help define their early biological processes. RESULTS: A transgenic a-myosin heavy chain (a-MHC) ES cell lineage was generated, expressing puromycin resistance and enhanced green fluorescent protein (EGFP) under the control of the a-MHC promoter. A puromycin-resistant, EGFP+, a-MHC+ cardiomyocyte population was isolated with over 92% purity. RNA was isolated after electrophysiological characterisation of the cardiomyocytes. Comprehensive transcriptome analysis of a-MHC+ cardiomyocytes in comparison to undifferentiated a-MHC ES cells and the control population from 15-day old embryoid bodies (EBs) led to the identification of 884 upregulated probe sets and 951 downregulated probe sets in a-MHC+ cardiomyocytes. A subset of upregulated genes encodes for cytoskeletal and voltage-dependent channel proteins, and proteins participating in aerobic energy metabolism. Interestingly, mitosis, apoptosis and Wnt signalling-associated genes were downregulated in the cardiomyocytes. In contrast, annotations for genes upregulated in the a-MHC+ cardiomyocytes are enriched for the gene ontology categories: enzyme-linked receptor protein signalling pathway (GO:0007167); protein kinase activity (GO:0004672); negative regulation of Wnt receptor signalling pathway (GO:0030178); regulation of cell size (O:0008361); as well as for the Biocarta p38 MAPK signalling pathway and KEGG calcium signalling pathway. CONCLUSION: The specific pattern of gene expression in the cardiomyocytes derived from ES cells reflects the biological, physiological and functional processes occurring in mature cardiomyocytes. Identification of cardiomyocyte-specific gene expression patterns and signalling pathways will contribute towards elucidating their roles during intact cardiac function.
Keywords:Apoptosis, Cell Differentiation, Cell Proliferation, Cluster Analysis, Embryonic Stem Cells, Gene Expression Profiling, Gene Expression Regulation, Genetic Engineering, Cardiac Myocytes, Oligonucleotide Array Sequence Analysis, Messenger RNA, Ventricular Myosins, Animals, Mice
Source:Genome Biology
Publisher:BioMed Central
Page Range:R56
Date:11 April 2007
Official Publication:https://doi.org/10.1186/gb-2007-8-4-r56
PubMed:View item in PubMed

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