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c-Met is essential for wound healing in the skin

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Item Type:Article
Title:c-Met is essential for wound healing in the skin
Creators Name:Chmielowiec, J. and Borowiak, M. and Morkel, M. and Stradal, T. and Munz, B. and Werner, S. and Wehland, J. and Birchmeier, C. and Birchmeier, W.
Abstract:Wound healing of the skin is a crucial regenerative process in adult mammals. We examined wound healing in conditional mutant mice, in which the c-Met gene that encodes the receptor of hepatocyte growth factor/scatter factor was mutated in the epidermis by cre recombinase. c-Met-deficient keratinocytes were unable to contribute to the reepithelialization of skin wounds. In conditional c-Met mutant mice, wound closure was slightly attenuated, but occurred exclusively by a few (5%) keratinocytes that had escaped recombination. This demonstrates that the wound process selected and amplified residual cells that express a functional c-Met receptor. We also cultured primary keratinocytes from the skin of conditional c-Met mutant mice and examined them in scratch wound assays. Again, closure of scratch wounds occurred by the few remaining c-Met-positive cells. Our data show that c-Met signaling not only controls cell growth and migration during embryogenesis but is also essential for the generation of the hyperproliferative epithelium in skin wounds, and thus for a fundamental regenerative process in the adult.
Keywords:Autocrine Communication, Cultured Cells, Hepatocyte Growth Factor, Integrases, Keratinocytes, Mutation, Proto-Oncogene Proteins c-met, Signal Transduction, Skin Physiology, Wound Healing, Knockout, Animals, Mice
Source:Journal of Cell Biology
Publisher:Rockefeller University Press
Page Range:151-162
Date:9 April 2007
Official Publication:https://doi.org/10.1083/jcb.200701086
PubMed:View item in PubMed

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