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Vaccination with autologous non-irradiated dendritic cells in patients with bcr/abl+ chronic myeloid leukaemia

Item Type:Article
Title:Vaccination with autologous non-irradiated dendritic cells in patients with bcr/abl+ chronic myeloid leukaemia
Creators Name:Westermann, J. and Kopp, J. and van Lessen, A. and Hecker, A.C. and Baskaynak, G. and le Coutre, P. and Doehner, K. and Doehner, H. and Doerken, B. and Pezzutto, A.
Abstract:In chronic myeloid leukaemia (CML), dendritic cells (DC) and leukaemic cells share a common progeny, leading to constitutive expression of putative tumour antigens, such as bcr/abl, in DC. In this phase-I/II study, autologous DC were used as a vaccine in patients with chronic phase bcr/abl+ CML, who had not achieved an adequate cytogenetic response after treatment with alpha-interferon or imatinib. Ten patients were enrolled, DC were generated from peripheral blood monocytes and vaccination consisted of four subcutaneous injections of increasing numbers of DC (1-50 x 10(6) cells per injection) on days 1, 2, 8 and 21. Vaccination was feasible and safe. Improvement of the cytogenetic/molecular response, as detected by fluorescence in situ hybridization of peripheral blood mononuclear cells (PBMC), was possibly related to vaccination in four of 10 patients. In three of these patients, T cells recognizing leukaemia-associated antigens became detectable. The proliferative capacity of PBMC in response to autologous DC increased after vaccination in all evaluable patients. We conclude that vaccination with autologous, non-irradiated leukaemic DC is feasible, safe and induces anti-leukaemic T-cell responses in some CML patients. DC vaccination might be useful in CML as postremission therapy, i.e. after treatment with tyrosine kinase inhibitors.
Keywords:Chronic myeloid leukaemia, Vaccination, Dendritic cells, T-cell immunity, bcr/abl
Source:British Journal of Haematology
Publisher:Blackwell Publishing
Page Range:297-306
Date:May 2007
Official Publication:https://doi.org/10.1111/j.1365-2141.2007.06547.x
PubMed:View item in PubMed

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