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The transcriptional repressor Glis2 is a novel binding partner for p120 catenin

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Item Type:Article
Title:The transcriptional repressor Glis2 is a novel binding partner for p120 catenin
Creators Name:Hosking, C.R. and Ulloa, F. and Hogan, C. and Ferber, E. and Figueroa, A. and Gevaert, K. and Birchmeier, W. and Briscoe, J. and Fujita, Y.
Abstract:Monitoring Editor: Richard Assoian In epithelial cells, p120 catenin localizes at cell-cell contacts and regulates adhesive function of the cadherin complex. In addition, p120 has been reported to localize in the nucleus, although the nuclear function of p120 is not fully understood. Here, we report the identification of Glis2 as a novel binding protein for p120. Glis2 is a Kruppel-like transcriptional repressor with homology to the Gli family, but its physiological function has not been well characterized. In this study we show that coexpression of Glis2 and Src induces nuclear translocation of p120. Furthermore, p120 induces the C-terminal cleavage of Glis2, and this cleavage is further enhanced by Src. The cleaved form of Glis2 loses one of its five zinc finger domains, but is still able to bind DNA. Functional studies in chick neural tube indicate that full-length Glis2 can affect neuronal differentiation, while the cleaved form requires coexpression of p120 to have a similar effect. These data indicate that p120 has additional novel functions in the nucleus together with Glis2.
Keywords:Cell Nucleus Active Transport, COS Cells, Cattle, Cell Adhesion Molecules, Cell Differentiation, Cell Line, Cercopithecus aethiops, DNA, Kruppel-Like Transcription Factors, Neurons, Phosphoproteins, Protein Binding, RNA Interference, Recombinant Fusion Proteins, Two-Hybrid System Techniques, Zinc Fingers, src-Family Kinases, Animals, Chick Embryo
Source:Molecular Biology of the Cell
Publisher:American Society for Cell Biology
Page Range:1918-1927
Date:1 May 2007
Official Publication:https://doi.org/10.1091/mbc.E06-10-0941
PubMed:View item in PubMed

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