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Histone acetylation increases chromatin accessibility

Item Type:Article
Title:Histone acetylation increases chromatin accessibility
Creators Name:Goerisch, S.M. and Wachsmuth, M. and Toth, K.F. and Lichter, P. and Rippe, K.
Abstract:In eukaryotes, the interaction of DNA with proteins and supramolecular complexes involved in gene expression is controlled by the dynamic organization of chromatin inasmuch as it defines the DNA accessibility. Here, the nuclear distribution of microinjected fluorescein-labeled dextrans of 42 kDa to 2.5 MDa molecular mass was used to characterize the chromatin accessibility in dependence on histone acetylation. Measurements of the fluorescein-dextran sizes were combined with an image correlation spectroscopy analysis, and three different interphase chromatin condensation states with apparent pore sizes of 16-20 nm, 36-56 nm and 60-100 nm were identified. A reversible change of the chromatin conformation to a uniform 60-100 nm pore size distribution was observed upon increased histone acetylation. This result identifies histone acetylation as a central factor in the dynamic regulation of chromatin accessibility during interphase. In mitotic chromosomes, the chromatin exclusion limit was 10-20 nm and independent of the histone acetylation state.
Keywords:Heterochromatin, Image correlation spectroscopy, Trichostatin A, Microinjection
Source:Journal of Cell Science
Publisher:Company of Biologists
Page Range:5825-5834
Date:15 December 2005
Official Publication:https://doi.org/10.1242/jcs.02689
PubMed:View item in PubMed

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