TRPV1 at nerve endings regulates growth cone morphology and movement through cytoskeleton reorganization

Item Type:	Article
Title:	TRPV1 at nerve endings regulates growth cone morphology and movement through cytoskeleton reorganization
Creators Name:	Goswami, C. and Schmidt, H. and Hucho, F.
Abstract:	While the importance of Ca2+ channel activity in axonal path finding is established, the underlying mechanisms are not clear. Here, we show that transient receptor potential vanilloid receptor 1 (TRPV1), a member of the TRP superfamily of nonspecific ion channels, is physically and functionally present at dynamic neuronal extensions, including growth cones. These nonselective cation channels sense exogenous ligands, such as resenifera toxin, and endogenous ligands, such as N-arachidonoyl-dopamine (NADA), and affect the integrity of microtubule cytoskeleton. Using TRPV1-transiently transfected F11 cells and embryonic dorsal root ganglia explants, we show that activation of TRPV1 results in growth cone retraction, and collapse and formation of varicosities along neurites. These changes were due to TRPV1-activation-mediated disassembly of microtubules and are partly Ca2+-independent. Prolonged activation with very low doses (1 nm) of NADA results in shortening of neurites in the majority of isolectin B4-positive dorsal root ganglia neurones. We postulate that TRPV1 activation plays an inhibitory role in sensory neuronal extension and motility by regulating the disassembly of microtubules. This might have a role in the chronification of pain.
Keywords:	Capsaicin, Cytoskeleton, Dorsal root ganglia (DRG), Growth cone, TRPV1
Source:	FEBS Journal
ISSN:	1742-464X
Publisher:	Blackwell Publishing
Volume:	274
Number:	3
Page Range:	760-772
Date:	February 2007
Official Publication:	https://doi.org/10.1111/j.1742-4658.2006.05621.x
PubMed:	View item in PubMed

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