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Genes from Chagas susceptibility loci that are differentially expressed in T. cruzi-resistant mice are candidates accounting for impaired immunity

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Item Type:Article
Title:Genes from Chagas susceptibility loci that are differentially expressed in T. cruzi-resistant mice are candidates accounting for impaired immunity
Creators Name:Graefe, S.E.B. and Streichert, T. and Budde, B.S. and Nuernberg, P. and Steeg, C. and Mueller-Myhsok, B. and Fleischer, B.
Abstract:Variation between inbred mice of susceptibility to experimental Trypanosoma cruzi infection has frequently been described, but the immunogenetic background is poorly understood. The outcross of the susceptible parental mouse strains C57BL/6 (B6) and DBA/2 (D2), B6D2F1 (F1) mice, is highly resistant to this parasite. In the present study we show by quantitative PCR that the increase of tissue parasitism during the early phase of infection is comparable up to day 11 between susceptible B6 and resistant F1 mice. A reduction of splenic parasite burdens occurs thereafter in both strains but is comparatively retarded in susceptible mice. Splenic microarchitecture is progressively disrupted with loss of follicles and B lymphocytes in B6 mice, but not in F1 mice. By genotyping of additional backcross offspring we corroborate our earlier findings that susceptibility maps to three loci on Chromosomes 5, 13 and 17. Analysis of gene expression of spleen cells from infected B6 and F1 mice with microarrays identifies about 0.3% of transcripts that are differentially expressed. Assuming that differential susceptibility is mediated by altered gene expression, we propose that the following differentially expressed transcripts from these loci are strong candidates for the observed phenotypic variation: H2-Ealpha, H2-D1, Ng23, Msh5 and Tubb5 from Chromosome 17; and Cxcl11, Bmp2k and Spp1 from Chromosome 5. Our results indicate that innate mechanisms are not of primary relevance to resistance of F1 mice to T. cruzi infection, and that differential susceptibility to experimental infection with this protozoan pathogen is not paralleled by extensive variation of the transcriptome.
Keywords:Animal Disease Models, Base Sequence, Chagas Disease, Chromosome Mapping, DNA Primers, Gene Expression, Genetic Predisposition to Disease, Host-Parasite Interactions, Inbred C57BL Mice, Inbred DBA Mice, Oligonucleotide Array Sequence Analysis, Species Specificity, Spleen, Time Factors, Trypanosoma cruzi, Animals, Mice
Source:PLoS ONE
Publisher:Public Library of Science
Page Range:e57
Date:20 December 2006
Official Publication:https://doi.org/10.1371/journal.pone.0000057
PubMed:View item in PubMed

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