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T cell homing to tumors detected by 3D-coordinated positron emission tomography and magnetic resonance imaging

Item Type:Article
Title:T cell homing to tumors detected by 3D-coordinated positron emission tomography and magnetic resonance imaging
Creators Name:Agger, R. and Petersen, M.S. and Petersen, C.C. and Hansen, S.B. and Stodkilde-Jorgensen, H. and Skands, U. and Blankenstein, T. and Andersen, T.E. and Hulgaard, E.F. and Jorgensen, J.T. and Marqversen, J. and Gundersen, H.J. and Hokland, M.E.
Abstract:A general hindrance to progress in adoptive cellular therapy is the lack of detailed knowledge of the fate of transferred cells in the body of the recipient. In this study, we present a novel technique for tracking of I-labeled cells in situ, which combines the high spatial resolution of magnetic resonance imaging with the high sensitivity and spatial accuracy of positron emission tomography. We have used this technique, together with determination of tissue radioactivity, flow cytometry, and microscopy, to characterize and quantitate the specific accumulation of transferred CD8 T cells in tumor tissue in a mouse model. Transgenic CD8 T cells, specific for the ovalbumin peptide SIINFEKL, were adoptively transferred to recipients carrying a subcutaneous tumor of the ovalbumin-expressing malignant melanoma cell line B16-OVA. The number of SIINFEKL-specific CD8 cells in the tumor tissue was determined by flow cytometry each day for 8 consecutive days after adoptive transfer. From low levels 1 day after injection, their number gradually increased until day 5 when an average of 3.3x10 SIINFEKL-specific cells per gram tumor tissue was found. By applying the combined positron emission tomography/magnetic resonance imaging technique we were able to determine the position of the transferred, I-labeled SIINFEKL-specific T cells in 3 dimensions in recipient mice, and could demonstrate a highly significant accumulation of the I label in and around the subcutaneous B16-OVA tumors compared with normal tissue. Accumulation of I was significantly higher in B16-OVA than in B16 tumors not expressing the OVA antigen.
Keywords:Adoptive Cell Transfer, T Cell Tracking, PET, MRI, Animals, Mice
Source:Journal of Immunotherapy
Publisher:Lippincott Williams & Wilkins (U.S.A.)
Page Range:29-39
Date:January 2007
Official Publication:https://doi.org/10.1097/01.cji.0000211326.38149.7e
PubMed:View item in PubMed

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