In vitro fertilization of bovine oocytes in protein-free culture system

Item Type:	Article
Title:	In vitro fertilization of bovine oocytes in protein-free culture system
Creators Name:	Smetanina, I.G. and Tatarinova, L.V. and Krivokharchenko, A.S.
Abstract:	We studied the possibility of fertilization of bovine oocyte-cumulus complexes, matured in vitro in a protein-free medium, in a protein-free culture system without preliminary capacitation of spermatozoa. The development of embryos to the morula-blastocyst and blastocyst stage was considered as a criterion of successful fertilization. It was shown that replacement of bovine serum albumin for polyvinyl alcohol or polyvinylpyrrolidone in Tyrode medium for fertilization did not affect significantly the development to the morula-blastocyst stage and the number of cells in blastocysts. It was also found that replacement bovine serum albumin for polyvinyl alcohol in all used media, Tyrode medium for washing of oocytes, medium for sperm preparation to fertilization, and Tyrode medium for fertilization, did not affect significantly the development to the morula-blastocyst and blastocyst stages, as well as on the number of cells in blastocysts. The results obtained suggest that in vitro fertilization of bovine oocyte-cumulus complexes is possible in a protein-free culture system without significant reduction in the capacity for in vitro development of the obtained embryos and number of cells in blastocysts.
Keywords:	Oocytes, Spermatozoa, In vitro fertilization, Cattle, Preimplantation embryos, Protein-free media, Animals
Source:	Russian Journal of Developmental Biology
ISSN:	1062-3604
Publisher:	Springer
Volume:	37
Number:	6
Page Range:	366-370
Date:	December 2006
Official Publication:	https://doi.org/10.1134/S106236040606004X
PubMed:	View item in PubMed

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